| 货号: S217848 |
| 产品全名: SPRY2 兔多抗 |
| 基因符号 IGAN3; hSPRY2 |
| UNIPROT ID: O43597 (Gene Accession – BC015745 ) |
| 背景: This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein. |
| 抗原: Fusion protein of human SPRY2 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 200-400;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Neuroscience, Cardiovascular, Signal Transduction, Stem Cells |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 217848(SPRY2 Antibody) at a dilution of 1/200(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the fusion protein and then with 217848(Anti-SPRY2 Antibody) at dilution 1/200. | 
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using 217848(Anti-SPRY2 Antibody) at a dilution of 1/200. | 
In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with fusion protein and then with D223217(Anti-SPRY2 Antibody) at dilution 1/200. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: A172, LOVO, A375, HepG2 cell lysates;
Primary antibody: 217848(SPRY2 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 40 seconds | |
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