| 货号: N261294 |
| 产品全名: Smad2/3 (6F7) 小鼠单抗 |
| 基因符号 SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3 |
| UNIPROT ID: P84022/Q15796 |
| 背景: Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 . Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses . |
| 抗原: Recombinant Protein of Smad3 |
| 经过测试的应用: IHC-P,WB |
| 推荐稀释比: WB: 1/500-1/1000 IHC: 1/50-1/100 |
| 种属反应性: Mouse |
| 克隆性： Mouse Monoclonal |
| 分子量: Calculated MW: 52 kDa; Observed MW: 52,60 kDa |
| 亚型: IgG |
| 纯化: Affinity Purified |
| 种属反应性: Human,Rat,Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| 研究领域: Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemical analysis of paraffin-embedded Human tonsils using Smad2/3 (6F7) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. | 
Immunohistochemistry analysis of paraffin-embedded Human Live rTissue using Smad2/3 (6F7) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. | 
Western blot analysis of Smad2/3 (6F7) in C6, MCF-7, Jurkat and 3T3 lysates using Smad2/3 (6F7) antibody | |
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