武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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RGS14 Rabbit pAb

Anti-RGS14 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S211491

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S211491
产品全名:   RGS14 兔多抗
基因符号   
UNIPROT ID:   O43566 (Gene Accession – BC014094 )
背景:   This gene encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
抗原:   Fusion protein of human RGS14
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 150-300;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 211491(RGS14 Antibody) at a dilution of 1/160(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 211491(Anti-RGS14 Antibody) at dilution 1/160.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Jurkat cell lysate;
Primary antibody: 211491(RGS14 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
           引用文献