武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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PYGL Rabbit pAb

Anti-PYGL Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S217725

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S217725
产品全名:   PYGL 兔多抗
基因符号   GSD6
UNIPROT ID:   P06737 (Gene Accession – BC082229 )
背景:   This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.
抗原:   Fusion protein of human PYGL
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Metabolism, Cancer
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 217725(PYGL Antibody) at a dilution of 1/80(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 217725(Anti-PYGL Antibody) at dilution 1/80.

The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 217725(Anti-PYGL Antibody) at a dilution of 1/80.

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with fusion protein and then with D222954(Anti-PYGL Antibody) at dilution 1/80.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Mouse liver tissue, 293T, PC3 cell lysates;
Primary antibody: 217725(PYGL Antibody) at dilution 1/1300;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds
           引用文献