武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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PPP1R12A Rabbit pAb

Anti-PPP1R12A Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S217626

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S217626
产品全名:   PPP1R12A 兔多抗
基因符号   MBS; M130; MYPT1
UNIPROT ID:   O14974 (Gene Accession – BC111752 )
背景:   Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC.
抗原:   Fusion protein of human PPP1R12A
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 25-100;WB: 1000-5000;ELISA: 2000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 217626(PPP1R12A Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 217626(Anti-PPP1R12A Antibody) at dilution 1/25.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: NIH/3T3 cells, A172 cells, hela cells, PC3 cells;
Primary antibody: 217626(PPP1R12A Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds
           引用文献