| 货号: S219468 |
| 产品全名: POLB 兔多抗 |
| 基因符号 |
| UNIPROT ID: P06746 (Gene Accession – BC106909 ) |
| 背景: The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011] |
| 抗原: Fusion protein of human POLB |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 100-300;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human brain tissue using 219468(POLB Antibody) at a dilution of 1/135(Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the fusion protein and then with 219468(Anti-POLB Antibody) at dilution 1/135. | 
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 219468(Anti-POLB Antibody) at a dilution of 1/135. | 
In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D227211(Anti-POLB Antibody) at dilution 1/135. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: K562, Hela cell lysates;
Primary antibody: 219468(POLB Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute | |
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