| 货号: S217697 |
| 产品全名: PLS3 兔多抗 |
| 基因符号 BMND18; T-plastin |
| UNIPROT ID: P13797 (Gene Accession – BC039049 ) |
| 背景: Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants. |
| 抗原: Fusion protein of human PLS3 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 217697(PLS3 Antibody) at a dilution of 1/75(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the fusion protein and then with 217697(Anti-PLS3 Antibody) at dilution 1/75. | 
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 217697(Anti-PLS3 Antibody) at a dilution of 1/75. | 
In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with fusion protein and then with D222885(Anti-PLS3 Antibody) at dilution 1/75. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: 293T and BGC-823 cell lysates;
Primary antibody: 217697(PLS3 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds | |
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