| 货号: S220331 |
| 产品全名: PIK3R3 兔多抗 |
| 基因符号 p55; p55-GAMMA |
| UNIPROT ID: Q92569 (Gene Accession – NP_001107644 ) |
| 背景: Phosphatidylinositol 3-kinase is a lipid kinase that phosphorylates the inositol ring of phosphatidylinositol and related compounds at the 3? position. PI 3-kinase p55 (PIK3R3) is comprised of a catalytic subunit and a regulatory subunit. The human p55 protein is composed of a rare amino terminal region followed by a proline-rich motif and two Src homology 2 (SH2) domains. PI 3-kinase p55 mRNAs are expressed in most human fetal and adult tissues; predominant expression is observed in the adult testis. Splice variant(s) of PI 3-kinase p55 have been identified; one of which has a deletion of 36 amino acids at the amino terminus and another which has an insertion of 59 amino acids at position 256 between the SH2 domains. Research suggests that PI 3-kinase p55 interacts with the IGFIR (Insulin-like growth factor-I receptor) and IR (Insulin receptor) and may be involved in PI 3-kinase activation by these receptors. |
| 抗原: Synthetic peptide of human PIK3R3 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Immunology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 220331(PIK3R3 Antibody) at a dilution of 1/25(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the synthetic peptide and then with 220331(Anti-PIK3R3 Antibody) at dilution 1/25. | 
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 220331(Anti-PIK3R3 Antibody) at a dilution of 1/25. | 
In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with synthetic peptide and then with D261373(Anti-PIK3R3 Antibody) at dilution 1/25. | 
Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Mouse brain tissue, human fetal brain tissue, mouse testis tissue, hela cells, human fetal kidney tissue;
Primary antibody: 220331(PIK3R3 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes | |
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