| 货号: N225495 |
| 产品全名: Phospho-B23 (Thr199) 兔多抗 |
| 基因符号 B23; NPM |
| UNIPROT ID: P06748 |
| 背景: Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029). |
| 抗原: The antiserum was produced against synthesized peptide derived from human NPM around the phosphorylation site of Thr199. AA range:171-220 |
| 经过测试的应用: WB,IHC-F,IHC-P,ICC/IF,ELISA |
| 推荐稀释比: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 分子量: Calculated MW: 33 kDa; Observed MW: 33 kDa |
| 亚型: IgG |
| 纯化: Affinity Chromatography |
| 种属反应性: Human, Mouse and Rat |
| Modification: Phosphorylated |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunofluorescence analysis of Phospho-B23 (Thr199) in HeLa treated with EGF using Phospho-B23 (Thr199) antibody. The picture on the right is blocked with the Phospho- peptide. | 
EnzymeLinked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phospho-peptide (Phospho-left) and NonPhospho-peptide (Phospho-right), using NPM (Phospho-Thr19antibody | 
Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using Phospho-B23 (Thr199) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right. | 
Western blot analysis of Phospho-B23 (Thr199) in HepG2 lysates using Phospho-B23 (Thr199) antibody.The lane on the right is blocked with the synthesized peptide. |
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