武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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NUP50 Rabbit pAb

Anti-NUP50 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S217659

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S217659
产品全名:   NUP50 兔多抗
基因符号   NPAP60; NPAP60L
UNIPROT ID:   Q9UKX7 (Gene Accession – BC016055 )
背景:   The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the Cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.
抗原:   Fusion protein of human NUP50
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-200;WB: 200-1000;ELISA: 1000-2000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction, Epigenetics and Nuclear Signaling, Cancer
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 217659(NUP50 Antibody) at a dilution of 1/30(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 217659(Anti-NUP50 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 217659(Anti-NUP50 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with fusion protein and then with D222796(Anti-NUP50 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Hela cells, 293T cells, A549 cells, HT29 cells, K562 cells;
Primary antibody: 217659(NUP50 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
           引用文献