武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

微信:Neweast Biosciences

咨询:027 – 63497286 

NF1 Rabbit pAb

Anti-NF1 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S221500

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S221500
产品全名:   NF1 兔多抗
基因符号   WSS; NFNS; VRNF
UNIPROT ID:   P21359 (Gene Accession – NP_000258 )
背景:   This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene.
抗原:   Synthetic peptide of human NF1
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 20-100;WB: 200-1000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction, Epigenetics and Nuclear Signaling, Cancer, Cardiovascular, Metabolism, Neuroscience
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 221500(NF1 Antibody) at a dilution of 1/25(Cytoplasm ).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the synthetic peptide and then with 221500(Anti-NF1 Antibody) at dilution 1/25.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: NIH/3T3 cell lysate;
Primary antibody: 221500(NF1 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 second
           引用文献