| 货号: S218285 |
| 产品全名: NELFA 兔多抗 |
| 基因符号 WHSC2; NELF-A; P/OKcl.15 |
| UNIPROT ID: Q9H3P2 (Gene Accession – BC002764 ) |
| 背景: This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. |
| 抗原: Fusion protein of human NELFA |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 100-300;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 218285(NELFA Antibody) at a dilution of 1/100(Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 218285(Anti-NELFA Antibody) at dilution 1/100. | 
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using 218285(Anti-NELFA Antibody) at a dilution of 1/100. | 
In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with fusion protein and then with D224094(Anti-NELFA Antibody) at dilution 1/100. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: A549 cell, Mouse testis tissue, Jurkat cell, RAW264.7 cell lysates;
Primary antibody: 218285(NELFA Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 15 seconds | |
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