| 货号: N263478 |
| 产品全名: NCF4 兔单克隆抗体 |
| 基因符号 NCF; CGD3; P40PHOX; SH3PXD4 |
| UNIPROT ID: Q15080 |
| 背景: The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
| 抗原: A synthetic peptide of human p40-phox |
| 经过测试的应用: WB,IHC-F,IHC-P,ICC/IF,IP |
| 推荐稀释比: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Monoclonal |
| 克隆编号: R02-3G7 |
| 分子量: Calculated MW: 39 kDa; Observed MW: 39 kDa |
| 亚型: IgG |
| 纯化: Affinity Purified |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| 研究领域: – |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin-embedded Human tonsil using p40phox antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. | 
Western blot analysis of p40phox in HL-60, U251 lysates using NCF4 antibody. | 
Immunocytochemistry analysis of p4phox (green) in hela using p4phox antibody,and DAPI(blue) | |
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