武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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LDHA Rabbit pAb

Anti-LDHA Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S222114

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S222114
产品全名:   LDHA 兔多抗
基因符号   LDHM; GSD11; PIG19; HEL-S-133P
UNIPROT ID:   P00338 (Gene Accession – NP_005557 )
背景:   The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.
抗原:   Synthetic peptide of human LDHA
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Metabolism, Cancer
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 222114(LDHA Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 222114(Anti-LDHA Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: NIH/3T3 and 231 cell lysates;
Primary antibody: 222114(LDHA Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 seconds
           引用文献