| 货号: S220124 |
| 产品全名: Lambda Light chain 兔多抗 |
| 基因符号 ?light chain |
| UNIPROT ID: P01701 (Gene Accession – P01701 ) |
| 背景: Antibody producing cells of the immune system require multiple rearrangements of immunoglobulin (antibody, Ig) genes. Immunoglobulins are four-chain, Y-shaped, monomeric structures of two identical heavy chains and two identical light chains held together through interchain disulfide bonds. Immunoglobulins in vertebrates help to remove non-self molecules or cells (antigens) by recognizing and binding to the antigen and carrying out effector functions that activate the immune system. Variable genetic combinations of the five heavy chain classes (M, D, G, E and A) and the two light chain isotypes, Kappa and Lambda, confer the role of an antibody. The variable region genes encoding immunoglobulin Kappa and Lambda chains are assembled from three DNA segments, the V, C and J genes. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. |
| 抗原: Synthetic peptide of human Lambda Light chain |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Immunology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human brain tissue using 220124(Lambda Light chain Antibody) at a dilution of 1/20(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the synthetic peptide and then with 220124(Anti-Lambda Light chain Antibody) at dilution 1/20. | 
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 220124(Anti-Lambda Light chain Antibody) at a dilution of 1/20. | 
In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with synthetic peptide and then with D260991(Anti-Lambda Light chain Antibody) at dilution 1/20. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Human plasma solution;
Primary antibody: 220124(Lambda Light chain Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds | |
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