武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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HADHA Rabbit pAb

Anti-HADHA Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S211322

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S211322
产品全名:   HADHA 兔多抗
基因符号   GBP; ECHA; HADH; LCEH; MTPA; LCHAD; TP-ALPHA
UNIPROT ID:   P40939 (Gene Accession – BC009235 )
背景:   This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.
抗原:   Fusion protein of human HADHA
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 25-100;WB: 1000-5000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Metabolism, Cancer, Cardiovascular
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 211322(HADHA Antibody) at a dilution of 1/35(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the fusion protein and then with 211322(Anti-HADHA Antibody) at dilution 1/35.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: HepG2, Jurkat, 293T, Hela, RAW264.7, K562, NIH/3T3 cell lysates;
Primary antibody: 211322(HADHA Antibody) at dilution 1/500;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
           引用文献