| 货号: S217503 |
| 产品全名: H2AC12 兔多抗 |
| 基因符号 H2AH; H2A/S; H2AFALii; HIST1H2AH; dJ86C11.1 |
| UNIPROT ID: Q96KK5 (Gene Accession – NP_542163 ) |
| 背景: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015] |
| 抗原: Fusion protein of human H2AC12 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 217503(H2AC12 Antibody) at a dilution of 1/40(Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 217503(Anti-H2AC12 Antibody) at dilution 1/40. | 
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 217503(Anti-H2AC12 Antibody) at a dilution of 1/40. | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D222480(Anti-H2AC12 Antibody) at dilution 1/40. | 
Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: Mouse liver tissue, HepG2 cells, 293T cells, Hela cells, Raji cells, A375 cells, K562 cells;
Primary antibody: 217503(H2AC12 Antibody) at dilution 1/450;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds | |
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