武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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GPT Rabbit pAb

Anti-GPT Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S219632

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S219632
产品全名:   GPT 兔多抗
基因符号   AAT1; ALT1; GPT1
UNIPROT ID:   P24298 (Gene Accession – NP_005300 )
背景:   This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.
抗原:   Synthetic peptide of human GPT
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Metabolism
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 219632(GPT Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 219632(Anti-GPT Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: A431 cells;
Primary antibody: 219632(GPT Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 minutes
           引用文献