武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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GCLC Rabbit pAb

Anti-GCLC Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S211955

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S211955
产品全名:   GCLC 兔多抗
基因符号   GCL; GCS; GLCL; GLCLC
UNIPROT ID:   P48506 (Gene Accession – BC022487 )
背景:   Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
抗原:   Fusion protein of human GCLC
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Metabolism, Cell Biology
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using 211955(GCLC Antibody) at a dilution of 1/35(Cytoplasm).

Immunohistochemistry analysis of paraffin-embedded Human colorectal cancer tissue using 211955(Anti-GCLC Antibody) at a dilution of 1/35.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Raw264.7, A549, Hepg2 and Jurkat cell lysates;
Primary antibody: 211955(GCLC Antibody) at dilution 1/800;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
           引用文献