武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

微信:Neweast Biosciences

咨询:027 – 63497286 

FAP Rabbit pAb

Anti-FAP Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S217428

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S217428
产品全名:   FAP 兔多抗
基因符号   FAPA; SIMP; DPPIV; FAPalpha
UNIPROT ID:   Q12884 (Gene Accession – BC026250 )
背景:   The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
抗原:   Fusion protein of human FAP
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction, Cancer, Cell Biology
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 217428(FAP Antibody) at a dilution of 1/80(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 217428(Anti-FAP Antibody) at dilution 1/80.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: 231 and HepG2 cell lysates;
Primary antibody: 217428(FAP Antibody) at dilution 1/400;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 90 seconds
           引用文献