| 货号: S219061 |
| 产品全名: ECH1 兔多抗 |
| 基因符号 HPXEL |
| UNIPROT ID: Q13011 (Gene Accession – BC011792 ) |
| 背景: This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. |
| 抗原: Fusion protein of human ECH1 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-300;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Metabolism, Cardiovascular |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 219061(ECH1 Antibody) at a dilution of 1/50(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 219061(Anti-ECH1 Antibody) at dilution 1/50. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Human fetal liver tissue, Hela, 293T, HepG2 and A549 cell lysates;
Primary antibody: 219061(ECH1 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 7 seconds | |
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