| 货号: S221415 |
| 产品全名: DUSP3 兔多抗 |
| 基因符号 VHR |
| UNIPROT ID: P51452 (Gene Accession – NP_004081 ) |
| 背景: The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. |
| 抗原: Synthetic peptide of human DUSP3 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-100;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Metabolism, Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 221415(DUSP3 Antibody) at a dilution of 1/40(Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 221415(Anti-DUSP3 Antibody) at dilution 1/40. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: A172, 293T and SKOV3 cell lysates;
Primary antibody: 221415(DUSP3 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes | |
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