武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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DOK3 Rabbit pAb

Anti-DOK3 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S216479

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S216479
产品全名:   DOK3 兔多抗
基因符号   DOKL
UNIPROT ID:   Q7L591 (Gene Accession – BC004564 )
背景:   DOK3 gene maps to chromosome 5q35.3. Dok3 was tyrosine phosphorylated by Src family members Lck, Fyn, and Lyn. Immunoprecipitation studies showed that Dok3 bound inhibitors SHIP and Csk but did not bind RasGAP. Dok3 binding to SHIP occurred via the SH2 domain. Dok3 also bound Csk via the Csk SH2 domain with possible involvement of the Csk SH3 domain as well.DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL1 function
抗原:   Fusion protein of human DOK3
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 216479(DOK3 Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the fusion protein and then with 216479(Anti-DOK3 Antibody) at dilution 1/25.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane: Human notum skin cancer tissue;
Primary antibody: 216479(DOK3 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
           引用文献