武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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DNAJA1 Rabbit pAb

Anti-DNAJA1 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S217523

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S217523
产品全名:   DNAJA1 兔多抗
基因符号   DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSPF4; NEDD7; hDJ-2
UNIPROT ID:   P31689 (Gene Accession – BC008182 )
背景:   DnaJ-like proteins interact with HSP 70 molecular chaperones and function to facilitate protein folding and mitochondrial protein import. HSP 40-4, also known as HDJ2, is the human DnaJ homolog that functions as a co-chaperone with a cysteine-rich zinc finger domain. The cellular redox enzyme thioredoxin interacts with HSP 40-4, and oxidation and reduction reversibly regulate HSP 40-4 function in response to the changing redox states of the cell. The zinc finger domain of HSP 40-4 may act as a redox sensor of chaperone-mediated protein-folding machinery, since HSP 40-4 inactivation leads to the oxidation of cysteine thiols and a simultaneous release of coordinated zinc. Loss of the HSP 40-4 protein may be linked to severe defects in spermatogenesis that involve aberrant androgen signaling.
抗原:   Fusion protein of human DNAJA1
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 217523(DNAJA1 Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 217523(Anti-DNAJA1 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 217523(Anti-DNAJA1 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D222514(Anti-DNAJA1 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: 231 cells, A431 cells, Raji cells, Jurkat cells, HepG2 cells;
Primary antibody: 217523(DNAJA1 Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
           引用文献