| 货号: S218584 |
| 产品全名: DDI2 兔多抗 |
| 基因符号 |
| UNIPROT ID: Q5TDH0 (Gene Accession – BC006011 ) |
| 背景: DDI1 and DDI2 are ubiquitin receptor homologs of the Saccharomyces cerevisiae ddi1 protein, which is involved in regulation of the cell cycle and the late secretory pathway. DDI2 is a 399 amino acid protein that contains one ubiquitin-like domain and exists as three isoforms as a result of alternative splicing. The gene encoding DDI2 maps to human chromosome 1, the largest human chromosome which spans about 260 million base pairs and makes up 8% of the human genome. Other notable genes located on chromosome 1 include LMNA, which is associated with the rare aging disease Hutchinson-Gilford progeria, and the MUTYH gene, which is partially responsible for familial adenomatous polyposis. Stickler syndrome, Parkinsons, Gaucher disease and Usher syndrome. |
| 抗原: Fusion protein of human DDI2 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-300;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 218584(DDI2 Antibody) at a dilution of 1/75(Cytoplasm and Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 218584(Anti-DDI2 Antibody) at dilution 1/75. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: HL60 and Jurkat cell lysates;
Primary antibody: 218584(DDI2 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds | |
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