| 货号: N262082 |
| 产品全名: Cullin 4A/4B 兔单克隆抗体 |
| 基因符号 CUL4B; KIAA0695; Cullin-4B; CUL-4B |
| UNIPROT ID: Q13620 |
| 背景: Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8. |
| 抗原: A synthetic peptide of human Cullin 4B |
| 经过测试的应用: WB,IHC-P |
| 推荐稀释比: WB: 1/500-1/1000 IHC: 1/50-1/100 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Monoclonal |
| 克隆编号: R06-3D3 |
| 分子量: Calculated MW: 104 kDa; Observed MW: 88,104 kDa |
| 亚型: IgG |
| 纯化: Affinity Purified |
| 种属反应性: Human,Hamster,Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| 研究领域: Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cullin 4A/B antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. | 
Western blot analysis of Cullin 4A/CUL4A+Cullin 4B/CUL4B in Jurkat, C6, CHO-K1 lysates using Cullin 4A/4B antibody. |
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