| 货号: N262062 |
| 产品全名: CPSF6 兔单克隆抗体 |
| 基因符号 CFIM; CFIM68; CFIM72; HPBRII-4; HPBRII-7 |
| UNIPROT ID: Q16630 |
| 背景: Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3′-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs (PubMed:9659921, PubMed:8626397, PubMed:14690600, PubMed:29276085). CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3′-end, so called cleavage and polyadenylation signals (pA signals) (PubMed:9659921, PubMed:8626397, PubMed:14690600). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3′-end formation (PubMed:23187700, PubMed:29276085). The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5′-UGUA-3′ elements localized in the 3′-untranslated region (UTR) for a huge number of pre-mRNAs (PubMed:20695905, PubMed:29276085). CPSF6 enhances NUDT21/CPSF5 binding to 5′-UGUA-3′ elements localized upstream of pA signals and promotes RNA looping, and hence activates directly the mRNA 3′-processing machinery (PubMed:15169763, PubMed:29276085, PubMed:21295486). Plays a role in mRNA export (PubMed:19864460). |
| 抗原: A synthetic peptide of human CPSF6 |
| 经过测试的应用: WB,IHC-F,IHC-P,ICC/IF,IP |
| 推荐稀释比: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Monoclonal |
| 克隆编号: R05-6K1 |
| 分子量: Calculated MW: 59 kDa; Observed MW: 70 kDa |
| 亚型: IgG |
| 纯化: Affinity Purified |
| 种属反应性: Human, Mouse and Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Western blot analysis of CPSF6 in rat Brain, MCF-7, 3T3, C6 lysates using CPSF6 antibody. |
