| 货号: N225413 |
| 产品全名: Cleaved-Caspase 6 p18 兔多抗 |
| 基因符号 CASP6; MCH2; Caspase-6; CASP-6; Apoptotic protease Mch-2 |
| UNIPROT ID: P55212 |
| 背景: Caspase-6 (Mch2) is one of the major executioner caspases functioning in cellular apoptotic processes. Upon apoptotic stimulation, initiator caspases such as caspase-9 are cleaved and activated. The activated upstream caspases further process downstream executioner caspases, such as caspase-3 and caspase-6, by cleaving them into large and small subunits, thereby initiating a caspase cascade leading to apoptosis. One of the major targets for caspase-6 is the membrane associated protein lamin A. |
| 抗原: The antiserum was produced against synthesized peptide derived from human Caspase 6. AA range:130-179 |
| 经过测试的应用: WB,IHC-P,ELISA |
| 推荐稀释比: WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 分子量: Calculated MW: 33 kDa; Observed MW: 20,35 kDa |
| 亚型: IgG |
| 纯化: Affinity Chromatography |
| 种属反应性: Human,Rat |
| Modification: Cleaved |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| 研究领域: apoptosis – Caspase family |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma tissue using Caspase 6 (Cleaved-Asp16, 2) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Sample with blocking peptide on the right. | 
Western blot analysis of Cleaved-Caspase 6 p18 in various lysates using Cleaved-Caspase 6 p18 antibody. | 
Western blot analysis of Cleaved-Caspase 6 p18 in 293 lysates using Cleaved-Caspase6 p18 (D179) antibody. | 
Western blot analysis of Cleaved-Caspase 6 p18 in 293 lysates treated with Etoposide using Cleaved-Caspase 6 p18 antibody.The lane on the right is blocked with the synthesized peptide. |
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