| 货号: S219941 |
| 产品全名: CKMT1A/CKMT1B 兔多抗 |
| 基因符号 CKMT; CKMT1; UMTCK |
| UNIPROT ID: P12532 (Gene Accession – NP_066270 ) |
| 背景: Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. |
| 抗原: Synthetic peptide of human CKMT1A/CKMT1B |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 40-200;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Metabolism, Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 219941(CKMT1A/CKMT1B Antibody) at a dilution of 1/55(Cytoplasm and Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 219941(Anti-CKMT1A/CKMT1B Antibody) at dilution 1/55. | 
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 219941(Anti-CKMT1A/CKMT1B Antibody) at a dilution of 1/55. | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with synthetic peptide and then with D260658(Anti-CKMT1A/CKMT1B Antibody) at dilution 1/55. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: MCF7 cell lysate;
Primary antibody: 219941(CKMT1A/CKMT1B Antibody) at dilution 1/600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute | |
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