武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

微信:Neweast Biosciences

咨询:027 – 63497286 

CDKN2B Rabbit pAb

Anti-CDKN2B Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S222402

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S222402
产品全名:   CDKN2B 兔多抗
基因符号   P15; MTS2; TP15; CDK4I; INK4B; p15INK4b
UNIPROT ID:   P42772 (Gene Accession – NP_004927 )
背景:   This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.
抗原:   Synthetic peptide of human CDKN2B
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Cancer, Epigenetics and Nuclear Signaling, Signal Transduction
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 222402(CDKN2B Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the synthetic peptide and then with 222402(Anti-CDKN2B Antibody) at dilution 1/50.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: 293T cell lysate;
Primary antibody: 222402(CDKN2B Antibody) at dilution 1/1350;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 40 seconds
           引用文献