| 货号: S216408 |
| 产品全名: CDK2 兔多抗 |
| 基因符号 p33(CDK2) |
| UNIPROT ID: P24941 (Gene Accession – BC003065 ) |
| 背景: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase comple,x whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. |
| 抗原: Fusion protein of human CDK2 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 200-1000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling, Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 216408(CDK2 Antibody) at a dilution of 1/30(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 216408(Anti-CDK2 Antibody) at dilution 1/30. | 
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 216408(Anti-CDK2 Antibody) at a dilution of 1/30. | 
In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with fusion protein and then with D220395(Anti-CDK2 Antibody) at dilution 1/30. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: 231 cells, NIH/3T3 cells, hela cells, human fetal liver tissue;
Primary antibody: 216408(CDK2 Antibody) at dilution 1/275;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute | |
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