| 货号: S220442 |
| 产品全名: CD47 兔多抗 |
| 基因符号 IAP; OA3; MER6 |
| UNIPROT ID: Q08722 (Gene Accession – NP_001768 ) |
| 背景: This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. |
| 抗原: Synthetic peptide of human CD47 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 1000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cancer, Immunology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human brain tissue using 220442(CD47 Antibody) at a dilution of 1/50(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the synthetic peptide and then with 220442(Anti-CD47 Antibody) at dilution 1/50. | 
The image on the left is immunohistochemistry of paraffin-embedded Human lymphoma tissue using 220442(Anti-CD47 Antibody) at a dilution of 1/50. | 
In comparision with the IHC on the left, the same paraffin-embedded Human lymphoma tissue is first treated with synthetic peptide and then with D261534(Anti-CD47 Antibody) at dilution 1/50. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Human metastatic poorly differentiated adenocarcinoma of the ovary tissue, Human ovarian cancer tissue lysate, Human ovarian cancer ( granulosa cell tumor ) tissue lysates;
Primary antibody: 220442(CD47 Antibody) at dilution 1/600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 3 minutes | |
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