武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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CD33 Rabbit pAb

Anti-CD33 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S217265

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S217265
产品全名:   CD33 兔多抗
基因符号   p67; SIGLEC3; SIGLEC-3
UNIPROT ID:   P20138 (Gene Accession – BC028152 )
背景:   Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
抗原:   Fusion protein of human CD33
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Immunology, Stem Cells
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 217265(CD33 Antibody) at a dilution of 1/30(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 217265(Anti-CD33 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: HepG2 cell, Mouse liver tissue, Jurkat cell lysates;
Primary antibody: 217265(CD33 Antibody) at dilution 1/750;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 3 minutes
           引用文献