| 货号: S219731 |
| 产品全名: CCND2 兔多抗 |
| 基因符号 KIAK0002 |
| UNIPROT ID: P30279 (Gene Accession – NP_001750 ) |
| 背景: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suY0ressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. |
| 抗原: Synthetic peptide of human CCND2 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 200-1000;ELISA: 1000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling, Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 219731(CCND2 Antibody) at a dilution of 1/25(Cytoplasm and Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 219731(Anti-CCND2 Antibody) at dilution 1/25. | 
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 219731(Anti-CCND2 Antibody) at a dilution of 1/25. | 
In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D260237(Anti-CCND2 Antibody) at dilution 1/25. | 
Gel: 10%SDS-PAGE, Lysate: 30 μg;
Lane: 231 cells;
Primary antibody: 219731(CCND2 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 minutes | |
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