| 货号: S218434 |
| 产品全名: CBFB 兔多抗 |
| 基因符号 PEBP2B |
| UNIPROT ID: Q13951 (Gene Accession – BC018509 ) |
| 背景: The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene. |
| 抗原: Full length fusion 蛋白 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 30-150;WB: 200-1000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 218434(CBFB Antibody) at a dilution of 1/30(Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the fusion protein and then with 218434(Anti-CBFB Antibody) at dilution 1/30. | 
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 218434(Anti-CBFB Antibody) at a dilution of 1/30. | 
In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D224424(Anti-CBFB Antibody) at dilution 1/30. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse lung tissue lysate;
Primary antibody: 218434(CBFB Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute | |
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