| 货号: S217230 |
| 产品全名: CAST 兔多抗 |
| 基因符号 BS-17 |
| UNIPROT ID: P20810 (Gene Accession – BC013579 ) |
| 背景: The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. |
| 抗原: Fusion protein of human CAST |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cell Biology, Neuroscience |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 217230(CAST Antibody) at a dilution of 1/60(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 217230(Anti-CAST Antibody) at dilution 1/60. | 
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 217230(Anti-CAST Antibody) at a dilution of 1/60. | 
In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with fusion protein and then with D222017(Anti-CAST Antibody) at dilution 1/60. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Lovo cells;
Primary antibody: 217230(CAST Antibody) at dilution 1/600;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 7 seconds | |
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