武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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CAPN2 Rabbit pAb

Anti-CAPN2 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S216381

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S216381
产品全名:   CAPN2 兔多抗
基因符号   CANP2; mCANP; CANPL2; CANPml
UNIPROT ID:   P17655 (Gene Accession – BC021303 )
背景:   The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5′ UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.
抗原:   Fusion protein of human CAPN2
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 100-300;WB: 1000-5000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction, Cancer, Cardiovascular, Metabolism, Cell Biology
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 216381(CAPN2 Antibody) at a dilution of 1/100(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 216381(Anti-CAPN2 Antibody) at dilution 1/100.

The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 216381(Anti-CAPN2 Antibody) at a dilution of 1/100.

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with fusion protein and then with D220329(Anti-CAPN2 Antibody) at dilution 1/100.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: A549, HepG2, Hela cell lysates;
Primary antibody: 216381(CAPN2 Antibody) at dilution 1/1200;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 seconds
           引用文献