| 货号: S221613 |
| 产品全名: BHLHA15 兔多抗 |
| 基因符号 MIST1; BHLHB8 |
| UNIPROT ID: Q7RTS1 (Gene Accession – NP_803238 ) |
| 背景: MIST1 (muscle, intestine and stomach expression 1), also known as BHLHB8 (basic helix-loop-helix domain containing, class B, 8), is a 189 amino acid nuclear protein expressed in liver, brain, skeletal muscle and spleen. MIST1 contains a basic helix-loop-helix (bHLH) domain and belongs to the bHLH family of transcription factors. Members of this family bind to the E-box motifs present in the promoter or enhancer regions of a variety of developmentally regulated genes and function as either transcriptional activators or transcriptional repressors. MIST1 is capable of binding to E-box motifs as a homodimer or a heterodimer with E-proteins (E12 and E47) and is believed to play a role regulating the transcriptional activity of MyoD, a protein involved in the regulation of muscle cell development. More specifically, MIST1 functions as a repressor of MyoD activity, ensuring that myoblast populations do not differentiate. In addition, MIST1 is expressed in mammary epithelial cells and is essential for the regulation of mammary gland development. |
| 抗原: Synthetic peptide of human BHLHA15 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 20-100;WB: 200-1000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 221613(BHLHA15 Antibody) at a dilution of 1/25(Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 221613(Anti-BHLHA15 Antibody) at dilution 1/25. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Human muscle tissue, Human spleen tissue, Mouse Pancreas tissue, Human pancreas tissue and Mouse liver tissue lysates;
Primary antibody: 221613(BHLHA15 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute | |
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