武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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BCL2 Rabbit pAb

Anti-BCL2 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S219669

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S219669
产品全名:   BCL2 兔多抗
基因符号   Bcl-2; PPP1R50
UNIPROT ID:   P10415 (Gene Accession – NP_000624 )
背景:   This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
抗原:   Synthetic peptide of human BCL2
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 30-150;WB: 1000-5000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Metabolism, Cancer, Cardiovascular
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 219669(BCL2 Antibody) at a dilution of 1/20(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 219669(Anti-BCL2 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 219669(Anti-BCL2 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with synthetic peptide and then with D260117(Anti-BCL2 Antibody) at dilution 1/20.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Jurkat, Raji, 293T, MCF-7 cell lysates;
Primary antibody: 219669(BCL2 Antibody) at dilution 1/1300;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
           引用文献