| 货号: S220666 |
| 产品全名: ANP32E 兔多抗 |
| 基因符号 LANPL; LANP-L |
| UNIPROT ID: Q9BTT0 (Gene Accession – NP_112182 ) |
| 背景: The Anp32 family consists of 32 evolutionarily-conserved proteins and is included within the superfamily of leucine-rich repeat (LRR) proteins. Leucine-rich acidic nuclear protein-like (LANPL), also called ANP32E or Cpd1, is a member of the Anp32 family. LANPL is located in the cytoplasm of peripheral blood leukocytes, colon, small intestine, prostate, thymus, spleen, skeletal muscle, liver and kidney. It has also been detected in the nucleus, cytoplasm and membrane of multiple brain regions. Upon phosphorylation, LANPL colocalizes and inhibits protein phosphatase 2A (PP2A), but does not inhibit PP1. LANPL modulates cell signalling and transduction of gene expression to regulate the morphology and dynamics of the cytoskeleton, cell adhesion, neural development and cerebellar morphogenesis. |
| 抗原: Synthetic peptide of human ANP32E |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cancer, Metabolism, Neuroscience |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 220666(ANP32E Antibody) at a dilution of 1/25(Cytoplasm and Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the synthetic peptide and then with 220666(Anti-ANP32E Antibody) at dilution 1/25. | 
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 220666(Anti-ANP32E Antibody) at a dilution of 1/25. | 
In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D261845(Anti-ANP32E Antibody) at dilution 1/25. | 
Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse thymus tissue;
Primary antibody: 220666(ANP32E Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds | |
|
