| 货号: N261403 |
| 产品全名: AKT 小鼠单抗 |
| 基因符号 AKT1 |
| UNIPROT ID: P31749/P31751/Q9Y243 |
| 背景: Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. |
| 抗原: Purified recombinant human AKT1 protein fragments expressed in E.coli.AKT1 interacts (via the C-terminus) with CCDC88A (via its C-terminus). Interacts with GRB10; the interaction leads to GRB10 phosphorylation thus promoting YWHAE-binding By similarity. Interacts with AGAP2 (isoform 2/PIKE-A); the interaction occurs in the presence of guanine nucleotides. Interacts with AKTIP. Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CDKN1B; the interaction phosphorylates CDKN1B promoting 14-3-3 binding and cell-cycle progression. Interacts with MAP3K5 and TRAF6. Interacts with BAD, PPP2R5B, STK3 and STK4. Interacts (via PH domain) with SIRT1. Interacts with SRPK2 in a phosphorylation-dependent manner. Interacts with RAF1. Interacts with TRIM13; the interaction ubiquitinates AKT1 leading to its proteasomal degradation. Interacts with TNK2 and CLK2. Interacts (via the C-terminus) with THEM4 (via its C-terminus). Interacts with and phosphorylated by PDPK1.AKT2 interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CLK2, PBH2 and TRAF6. AKT3 interacts (via PH domain) with TCL1A; this enhances AKT3 phosphorylation and activation. Interacts with TRAF6. |
| 经过测试的应用: WB,IP |
| 推荐稀释比: WB: 1/500-1/1000 IP: 1/20 |
| 种属反应性: Mouse |
| 克隆性： Mouse Monoclonal |
| 克隆编号: 3B11-G8-B1 |
| 分子量: Calculated MW: 56 kDa; Observed MW: 60 kDa |
| 亚型: IgG1 |
| 纯化: Affinity Purified |
| 种属反应性: Human, Mouse and Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| 研究领域: Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunoprecipitation analysis of AKT in Hela lysates using AKT(pan) antibody. | 
Western blot analysis of total AKT in 3T3, 293T, PC-12 and K562 lysates using AKT(pan) antibody. | 
Western blot analysis of AKT1, AKT2, AKT3 and DSBA recombinant antigen using DSBA antibody,and(Right)AKT1, AKT2 and AKT3 recombinant antigen fragments using AKT(pan) antibody. | |
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