| 货号: S215535 |
| 产品全名: ADORA2A 兔多抗 |
| 基因符号 A2aR; RDC8; ADORA2 |
| UNIPROT ID: P29274 (Gene Accession – NP_000666 ) |
| 背景: This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013] |
| 抗原: Synthetic peptide of human ADORA2A |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-100;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Cancer, Neuroscience, Cardiovascular |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human ovarian cancer using 215535(ADORA2A Antibody) at a dilution of 1/40(Cytoplasm and Cell membrane). | 
In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer is first treated with the synthetic peptide and then with 215535(Anti-ADORA2A Antibody) at dilution 1/40. | 
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer using 215535(Anti-ADORA2A Antibody) at a dilution of 1/40. | 
In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer is first treated with synthetic peptide and then with D163521(Anti-ADORA2A Antibody) at dilution 1/40. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse liver tissue lysate;
Primary antibody: 215535(ADORA2A Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 minutes | |
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