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6XHis Tag Rabbit pAb

Anti-6XHis Tag Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S222461

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S222461
产品全名:   6?His Tag 兔多抗
基因符号   
UNIPROT ID:   
背景:   The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification. It can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting. The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.
抗原:   Synthetic peptide: 18?His conjugated to KLH
经过测试的应用:   WB
推荐稀释比:    WB: 2000-10000;
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Fusion proteins containing the HIS Tag
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Cell Biology
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Gel: 8%SDS-PAGE, Lysate: 0.5/0.25/0.5/0.25 μg;
Lane 1-4: 0.5/0.25/0.5/0.25 μg Fusion protein containing the HIS Tag;
Primary antibody: 222461(6?His Tag Antibody) at dilution 1/2000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 seconds
           引用文献