武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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5T4 (DM137) Rabbit mAb

Anti-5T4 (DM137) Rabbit Monoclonal Antibody
5/5

¥445.00

货号:28482

规格: 100 μg

库存状态:In Stock

           产品描述          
货号:   28482
产品全名:   5T4(DM137) 兔单克隆抗体
基因符号   5T4
描述:   5T4 antibody(DM137) 兔单克隆抗体
背景:   This gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. The encoded protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is associated with poor clinical outcome in numerous cancers. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein.
经过测试的应用:   ELISA; Flow Cyt
推荐稀释比:   ELISA 1:5000-10000; Flow Cyt 1:100
种属反应性:   Rabbit
亚型:   Rabbit IgG
纯化:   Purified from cell culture supernatant by affinity chromatography
种属反应性:   人 5T4
成分:   Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
储存和运输:   Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human 5T4 protein, His tagged protein 11232 can bind Rabbit anti-5T4 monoclonal antibody (clone: DM137) in a linear range of 0.1-16 ng/ml.

Figure 2. 5T4 protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-5T4 (DM137) on Expi293 cells transfected with human 5T4 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram),and  Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram)
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