| 货号: 28381 |
| 产品全名: 2B4(DM70) 兔单克隆抗体 |
| 基因符号 CD244;2B4;SLAMF4;NKR2B4;NAIL;h2B4 |
| 描述: 2B4 antibody(DM70) 兔单克隆抗体 |
| 背景: This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| 经过测试的应用: ELISA; Flow Cyt |
| 推荐稀释比: ELISA 1:5000-10000; Flow Cyt 1:100 |
| 种属反应性: Rabbit |
| 亚型: Rabbit IgG |
| 纯化: Purified from cell culture supernatant by affinity chromatography |
| 种属反应性: 人 2B4 |
| 成分: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). |
Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human 2B4 protein, mFc-His tagged protein 11138][getskuurl sku=)] can bind Rabbit anti-2B4 monoclonal antibody ( clone: DM70) in a linear range of 1-100 ng/ml. | 
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human 2B4 (B) were surface stained with Rabbit anti-2B4 monoclonal antibody 1µg/ml ( clone: DM70) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody. | 
Figure 3. Flow cytometry data of serially titrated Rabbit anti-2B4 monoclonal antibody ( clone: DM70) on THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. | 
Figure 4. Affinity ranking of different Rabbit anti-2B4 mAb clones by titration of different concentration onto THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. |
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