| 货号: N226079 |
| 产品全名: MonoMethyl-Histone H3 (Lys4) 兔多抗 |
| 基因符号 H3K4me; H3 histone; HIST1H3A; Histone cluster 1; H3a |
| UNIPROT ID: Q16695 |
| 背景: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
| 抗原: A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3 |
| 经过测试的应用: WB,IHC-F,IHC-P,ICC/IF |
| 推荐稀释比: WB: 1/500-1/1000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 分子量: Calculated MW: 16 kDa; Observed MW: 16 kDa |
| 亚型: IgG |
| 纯化: Affinity Purified |
| 种属反应性: Human, Mouse and Rat |
| Modification: Methylated |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
MonoMethyl-Histone H3-K4 antibody Dotblot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K4 antibody. | 
Western blot analysis of MonoMethyl-Histone H3 (Lys4) in various cell lines lysates using MonoMethyl-Histone H3-K4 antibody. | 
Immunohistochemistry analysis of paraffin-embedded rat spleen using MonoMethyl-Histone H3-K4 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. | 
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using MonoMethyl-Histone H3-K4 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. | 
Chromatin Immunoprecipitation MonoMethyl-Histone H3-K4 pAb Chromatin immunoprecipitation analysis of extracts of 293T cells, using MonoMethyl-Histone H3-K4 antibody and rabbit IgG. P1 and P2 were located on promoter (GAPDH). The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the rat ios of the immunoprecipitated DNA to the input. | |
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