| 货号: N225424 |
| 产品全名: Phospho-Cardiac Troponin I (Ser22/Ser23) 兔多抗 |
| 基因符号 TNNI3; TNNC1; Troponin I; cardiac muscle; Cardiac troponin I |
| UNIPROT ID: P23693 |
| 背景: Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: tnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). |
| 抗原: The antiserum was produced against synthesized peptide derived from rat TNNI3 around the phosphorylation site of Ser22 and Ser23. AA range:5-54 |
| 经过测试的应用: WB,IHC-P,ELISA |
| 推荐稀释比: WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 分子量: Calculated MW: 24 kDa; Observed MW: 28 kDa |
| 亚型: IgG |
| 纯化: Affinity Chromatography |
| 种属反应性: Mouse,Rat |
| Modification: Phosphorylated |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| 研究领域: Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
EnzymeLinked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phospho-peptide (Phospho-left) and NonPhospho-peptide (Phospho-right), using TNNI3 (Phospho-Ser22+Ser23) antibody. | 
Western blot analysis of Phospho-Cardiac Troponin I (Ser22/Ser23) in SH-SY5Y lysates using Phospho-Cardiac Troponin I (Ser22/Ser23) antibody. | 
Immunohistochemistry analysis of paraffin-embedded Human skeletal muscle using TNNI3 (Phospho-Ser22+Ser23) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right. | 
Western blot analysis of Phospho-Cardiac Troponin I (Ser22/Ser23) in mouse heart lysates using Phospho-Cardiac Troponin I (Ser22/Ser23) antibody.The lane on the right is blocked with the Phospho- peptide. |
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