武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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CHFR Rabbit pAb

Anti-CHFR Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S221789

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S221789
产品全名:   CHFR 兔多抗
基因符号   RNF116; RNF196
UNIPROT ID:   Q96EP1 (Gene Accession – NP_001154816 )
背景:   This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described.
抗原:   Synthetic peptide of human CHFR
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 20-100;WB: 200-1000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Epigenetics and Nuclear Signaling, Cancer
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 221789(CHFR Antibody) at a dilution of 1/20(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the synthetic peptide and then with 221789(Anti-CHFR Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: Hela, Jurkat, A549, HepG2, 293T and 231 cell lysates;
Primary antibody: 221789(CHFR Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
           引用文献