| 货号: S221618 |
| 产品全名: BLVRB 兔多抗 |
| 基因符号 FLR; BVRB; SDR43U1; HEL-S-10 |
| UNIPROT ID: P30043 (Gene Accession – NP_000704 ) |
| 背景: BLVRB (biliverdin reductase B or BVR-B), also known as flavin reductase (FR), NADPH-dependent diaphorase, Biliverdin-IX ?-reductase or green heme-binding protien (GHBP) is an enzyme involved in fetal heme metabolism. It is dependent on NADPH and is responsible for catalyzing the transfer of electrons to flavins from reduced pyridine nucleotides. BLVRB exists as a monomer, localizes to the cytoplasm and is highly expressed in fetal liver and adult erythrocytes and, to a lesser extent, in heart, lung, cerebrum and adrenal gland. In liver, BLVRB functions to convert biliverdin (isoforms IX?, IX? and IX?) to bilirubin. BLVRB contains one binding site for all of its substrates and predominantly interacts with them through hydrophobic interactions. BLVRB also exhibits ferric reductase activity. In addition, it is commonly used as a reliable marker for NOS. |
| 抗原: Synthetic peptide of human BLVRB |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 40-200;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Metabolism |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 221618(BLVRB Antibody) at a dilution of 1/65(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the synthetic peptide and then with 221618(Anti-BLVRB Antibody) at dilution 1/65. | 
The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 221618(Anti-BLVRB Antibody) at a dilution of 1/65. | 
In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with synthetic peptide and then with D263280(Anti-BLVRB Antibody) at dilution 1/65. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: A549 and K562 cell lysate;
Primary antibody: 221618(BLVRB Antibody) at dilution 1/600;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds | |
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