| 货号: S221524 |
| 产品全名: ATP1B2 兔多抗 |
| 基因符号 AMOG |
| UNIPROT ID: P14415 (Gene Accession – NP_001669 ) |
| 背景: The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 2 subunit. Two transcript variants encoding different isoforms have been found for this gene. |
| 抗原: Synthetic peptide of human ATP1B2 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-100;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Metabolism |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 221524(ATP1B2 Antibody) at a dilution of 1/25(Cell membrane). | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the synthetic peptide and then with 221524(Anti-ATP1B2 Antibody) at dilution 1/25. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Human cerebella tissue lysate;
Primary antibody: 221524(ATP1B2 Antibody) at dilution 1/650;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 minutes | |
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